Ivan Franjic will play no further part in Australia's World Cup campaign after sustaining a hamstring injury in the 3-1 defeat to Chile on Friday night.
The 26-year-old lasted a little over 45 minutes before being replaced, with Socceroos coach Ange Postecoglou confirming that the defender's scans had shown a "fairly significant tear".
Prior to being substituted Franjic had played a prominent role in helping Australia get back into the contest, crossing for Tim Cahill who halved the deficit to just one goal.
The Aussies' remaining Group B games come against the Netherlands and Spain respectively.