Barnsley take on another Championship high-flyer after moving out of the relegation places with the win at home to Brighton & Hove Albion in midweek.
Watford themselves were beaten for the first time in eight league matches when they went down 2-1 at home to Blackpool.
Aside from the injured Scott Golbourne, the hosts have an almost full-strength squad to choose from for the second home game running.
Gianfranco Zola has a number of players at risk of suspension but is unlikely to make drastic changes to his side despite their defeat last time, and Manuel Almunia could return in goal.
Recent form
Barnsley: WWLLDW
Watford: WWWDWL
Possible starting lineups
Barnsley: Steele; Delap, Wiseman, Cranie, Kennedy; O'Brien, Etuhu, Perkins, Mellis; Scotland, O'Grady
Watford: Almunia; Doyley, Nosworthy, Briggs; Yeates, Pudil, Anya, Abdi, Hogg; Battochio, Deeney
Sports Mole says: 1-2