Great Britain's Ellie Simmonds failed to claim gold on day one of the IPC Swimming World Championships in Glasgow.
The four-time Paralympic champion was forced to settle for silver after being beaten by Ukraine's Yelyzaveta Mereshko in the S6 400m freestyle final on Monday.
"It was such a tough race," Simmonds, who had won every major title in that specific race since Beijing 2008, told BBC Sport. "I gave it the best I could, it just wasn't good enough to win, but it is all about next year and Rio [Paralympics]."
Overall, Britain claimed six medals on the opening day, with Andrew Mullen setting a new European record in the heats of the S5 50m breaststroke, but he finished second to Brazil's Daniel Dias in the final.