The Extra Preliminary Round FA Cup tie between Ascot United and Wembley FC on Friday will become the first live football match to be screened on Facebook.
Competition sponsors Budweiser agreed a deal with both non-league clubs earlier this week to screen the game from Ascot's 1,150-capacity ground.
"To be involved in the first game of the FA Cup this season is an honour in itself, but to be part of a world first and have our match broadcast to a massive global audience is fantastic for our club," Ascot United chairman Mike Harrison told The Guardian.
Budweiser have confirmed that only those over the legal drinking age of 18 will be able to view the match, which kicks off at 7.45pm.